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Primary antibodies, secondary antibodies and isotype controls

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Primary Antibodies, Secondary Antibodies, and Isotype Controls From R&D Systems

An antibody is a Y shaped protein that is produced in the immune system and is designed to target a specific protein or molecule. Due to the highly specific nature of antibodies, they can be used as powerful tools for research. Over the years a variety of techniques and applications have been developed using antibodies. These include Immunoassays, Flow Cytometry, Immunohistochemistry, Western blotting and more.

R&D Systems offers a wide range of antibodies to cytokines, adhesion molecules, proteases, neurotrophic factors, stem cell factors, signal transduction molecules, and developmental proteins. Monoclonal and polyclonal antibodies are available with multiple labeled options or unlabeled. With over 20,000 antibody products validated in 25+ species and 15+ applications, our antibodies fit into all work flows.

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Primary Antibodies

Primary antibodies are used to detect a wide range of targets in variety of applications. R&D Systems offers an extensive catalog of primary antibodies to take your research to the next level. We offer traditional monoclonal antibodies and polyclonal antibodies as well as recombinantly expressed antibodies. We provide multiple size options from our sample size to test an antibody to our ability to scale up antibody product and provide large quantity bulk sizes.

Browse All Primary Antibodies

Recombinant Monoclonal Antibodies
Minimize one source of experimental variability with recombinant antibodies. We offer recombinant rabbit monoclonal antibodies as well as recombinant mouse and rat antibodies from our traditional hybridomas.

LlaMABody™-Camelid Antibodies
Due to their small size, camelid antibodies are able to detect certain epitopes that may not be accessible with a traditional antibody. We offer VHH antibody domains, VHH domains on a human IgG scaffold, and native llama heavy chain IgG antibodies.

Biosimilar Antibodies
Our Biosimilar Antibodies are recombinantly expressed using the same sequence as the Therapeutic antibody. They are a great option for research use.

Conjugated Antibodies
Choose from our wide range of conjugation options. With over 15+ enzymatic and fluorescent conjugates analyze multiple targets with ease.

Custom Antibody Development
Utilize our expertise in cloning antigens and antibody engineering processes to create the antibody of your choice.

Epitope Tag Antibodies
Simplify your protein detection, identification, and purification experiments with our epitope tag antibodies.

 

Secondary Reagents & Isotype Controls

R&D Systems offers an assortment of secondary reagents and controls for flow cytometry, immunohistochemistry, immunocytochemistry, and Western blotting.

Secondary Antibodies
Species-specific secondary antibodies available in a variety of conjugated labels.

Isotype Controls
Options that cover multiple species, isoforms, and conjugates to match your primary antibody.

NorthernLights™ Fluorescent Secondary Antibodies
NorthernLight conjugated secondary antibodies are bright and resistant to photobleaching.

Cell and Tissue Staining Kits
Designed to optimize staining on cryostat, paraffin-embedded, and free-floating tissue sections. All the reagents required to maximize your IHC experiments.

VisUCyte™ HRP Polymer
Get cleaner and faster IHC results with a biotin-free detection reagent that eliminates the need to perform additional quenching of endogenous biotin and avidin.

 

Antibody Applications

By testing and validating our antibodies in-house we are able to stand behind our products and guarantee them in their stated applications.

Flow Cytometry Antibodies
Conjugated antibodies for surface and intracellular staining.

CyTOF Ready Antibodies
Take advantage of our large selection of antibodies that are ready to be used in CyTOF.

IHC and ICC Antibodies
Highly specific antibodies for Immunohistochemistry and Immunocytochemsitry.

Blocking/Neutralizing Antibodies
Our extensive collection of proteins and our bioassay expertise allow us to provide the best and most dependable selection of blocking/neutralizing antibodies on the market.

Western Blot Antibodies
View the full list of validated antibodies for Western blot.

Simple Western™ Certified Antibodies
A user-interactive database of antibodies validated for Simple Western assays. Submit new antibody data and receive a free separation or detection module.

Matched Antibody Pairs for ELISA
Qualified capture and detection antibody pairs for assay development on various assay platforms.

 

Antibody Quality and Reproducibility

R&D Systems carefully tests every antibody we produce to ensure outstanding performance. Our commitment and approach to quality allows you to be confident in your results and help you generate publication-quality data.

Built for Reproducibility
Results are everything, trust our antibodies. Learn about our commitment to antibody reproducibility.

Knockout Validated Antibodies
Specificity that has been confirmed with knockout cell lysates.

Customer Guarantee
Learn how our attention to quality allows us to offer a 100% guarantee on all our products and provide first class customer support.

Dual ISH-IHC Validation
By combining tissue staining strategies from Advanced Cell Diagnostics and R&D Systems, we are taking a multiomics approach to antibody validation.

 

Antibody Protocols

R&D Systems laboratories are pleased to share their in-house protocols. View what conditions we use to validated and test our antibodies

View all R&D Systems Protocols

Flow Cytometry Protocols
Protocols designed for staining membrane-associated proteins and intracellular molecules.

IHC and ICC Protocols
Learn to stain paraffin-embedded and frozen tissue with our IHC protocols and visualize your cells with our ICC protocols.

Western Blot Protocols
Use our in-house Western blot protocols to identify your next protein.

 

 

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Flow Cytometry Training Webinar

In this series of webinars, we will provide investigators with the necessary tools to design, run, and analyze a multi-parameter flow cytometric experiment. Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using fluorescently labeled antibodies. In order to maximize the quality of results obtained, researchers should become familiar with the basics when setting up an experiment.

View Flow Cytometry Training Webinars
Flow Cytometry Training Webinar

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Lessons from 20 Years in Antibody Validation and Reproducibility

Learn more about the R&D Systems approach to developing antibodies. This webinar highlights the importance of having an integrated approach to antibody development, which includes antigen design, testing, and quality control. Using examples for Western Blot, flow cytometry, and other major applications we will show some pitfalls and improvements in our 20 years of experience in developing new reagents.

View Antibody Validation and Reproducibility Webinar
Lessons from 20 Years in Antibody Validation and Reproducibility

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Validating Antibodies to Support the AstraZeneca Drug Discovery Pipeline

This webinar will outline our strategies for ensuring appropriate antibody selection and validation, comprising careful assessment of antibody specificity, selectivity and reproducibility. Case studies are presented to demonstrate how we apply our expertise and knowledge to deliver fit-for-purpose antibodies across AstraZeneca’s drug discovery projects.

View Drug Discovery Projects Webinar
Validating Antibodies to Support the AstraZeneca Drug Discovery Pipeline

Antibody Additional Resources

Posters

A Look Inside A Tumor
Metabolic Syndrome Signaling
Mona Lisa IHC/ICC Mosaic Wall Poster
American Gothic IHC/ICC Mosaic Wall Poster
Advantages of Recombinant Antibody Engineering

 

 

Product Brochures

Custom Reagents & Contract Services
Flow Cytometry eHandbook
Immunohistochemistry (IHC) Handbook
IHC/ICC Protocol Guide
Products for Immunology Research
CD4+ T Cell Subsets
A Guide to Human Immune Cell Characterization by Flow Cytometry
Neuroinflammation

 

Blog

Anti-idiotype Antibodies: Monoclonal Therapeutic Discovery and Development
ROR gamma t and Th17 Cells: New Highly-specific Antibody Will Find What You Have Been Missing

 

 

Literature

Dual ISH-IHC Antibody Validation
Development of Validated Monoclonal Antibodies
Phenotypic Characterization of Human M1 and M2a Macrophages
A Novel Rabbit Monoclonal Antibody Against the N-terminus of the Rat μ Opioid Receptor (OPRM1)

 

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Related Information

Antibody Matchmaker
Antibody Production Custom Quote
Custom Antibody Services
Custom Reagents & Contract Services
Immunohistochemistry Troubleshooting Guide